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Analysis of EIF4G2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2399-LGZ)

Introduction

Official Full Name: eukaryotic translation initiation factor 4 gamma 2<br />Also known as: P97; AAG1; DAP5; NAT1<br />Translation initiation is mediated by cap-specific recognition by eukaryotic translation initiation factor 4F (eIF4F), a cap-binding protein complex consisting of three subunits: eIF4A, eIF4E, and eIF4G. The protein encoded by this gene has similarity to the c-terminal region of eIF4G, which contains the binding sites of eIF4A and eIF3; in addition, eIF4G contains the binding site of eIF4E at the n-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product acts as a general repressor of translation by forming a translationally inactive complex. In vitro and in vivo studies have shown that translation of this mRNA is initiated only at non-aug (GUG) codons. Alternative splicing transcript variants encoding different isoforms of this gene have been described.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements