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Analysis of ENSA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0927-LGZ)

Introduction

Official Full Name: endosulfine alpha<br />Also known as: ARPP-19e<br />The protein encoded by this gene belongs to the highly conserved camp-regulated phosphorylation protein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, located on the plasma membrane of pancreatic β cells, and plays a key role in controlling the insulin release of pancreatic β cells. This protein is considered an endogenous regulator of KATP channels. In vitro studies have shown that this protein regulates insulin secretion through the interaction with the KATP channel, and this gene is considered as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encode distinct isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements