Analysis of EPHA2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1316-LGZ)

Introduction

Official Full Name: EPH receptor A2
Also known as: ECK; CTPA; ARCC2; CTPP1; CTRCT6
This gene belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors are involved in mediating developmental events, especially in the nervous system. Receptors in the EPH subfamily typically possess a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ephrin receptors are divided into two classes based on the similarity of their extracellular domain sequences and their binding affinity for ephrin-a and ephrin-b ligands. This gene encodes a protein that binds the ephrin-A ligand. Mutations in this gene are responsible for certain inherited cataract diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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