Analysis of Epizootic Hemorrhagic Disease Virus (EHDV) by Real-Time PCR Method (CAT#: STEM-MB-3141-LGZ)

Introduction

Epizootic haemorrhagic disease virus (EHDV) mainly infects deer, but sheep and cattle can also be infected. It belongs to the family Reoviridae, genus Orbivirus, and 8 or more serotypes have been found. EHDV has a double layered capsid which encases 10 segments of double stranded RNA.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.