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Analysis of EPO (Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0155-LGZ)

Introduction

Erythropoietin (EPO), also known as erythropoietin, hematopoietin, or hematopoietin, is a glycoprotein cytokine secreted primarily by the kidney in response to cellular hypoxia. It stimulates the production of red blood cells (erythropoiesis) in the bone marrow. Low levels of EPO (approximately 10 mU/mL) are constantly secreted and are sufficient to compensate for normal red blood cell turnover. Common causes of cellular hypoxia that result in elevated EPO levels (up to 10,000 mU/mL) include any anemia, as well as hypoxemia due to chronic lung disease.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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