Analysis of Equine Herpesvirus 2 (EHV-2) by Real-Time PCR Method (CAT#: STEM-MB-3172-LGZ)

Introduction

Equid herpesvirus 2 can be detected by PCR (or virus isolation) in nasal swab filtrates or from buffy coat cells of most adult horses. Horses may be infected in the first weeks of life, even in the presence of maternal antibody.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.