Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of ESRRG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0952-LGZ)

Introduction

Official Full Name: estrogen related receptor gamma<br />Also known as: ERR3; ERRg; NR3B3; ERRgamma; ERR-gamma<br />This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share a nearly identical DNA-binding domain consisting of two C4-type zinc finger motifs. ESRR members are orphan nuclear receptors; they bind to the estrogen response element and the steroidogenic factor 1 response element, activating genes controlled by both response elements in the absence of ligand. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, common regulators and promoters, and by targeting the same set of genes, esrr appears to interfere with ER-mediated estrogen responses in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen s signaling in breast cancer , and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified that differ predominantly at the 5' end, some of which encode protein isoforms that differ at the N terminus.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements