Analysis of ETNK2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0951-LGZ)

Introduction

Official Full Name: ethanolamine kinase 2
Also known as: EKI2; HMFT1716
The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of DCST2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0893-LGZ)
Analysis of TIGD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1780-LGZ)
Analysis of Macrococcus Caseolyticus by Real-Time PCR Method (CAT#: STEM-MB-3504-LGZ)
Analysis of RO60 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0643-LGZ)
Analysis of MIR29B2CHG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0445-LGZ)
Analysis of Everglades Virus (EVE) by Real-Time PCR Method (CAT#: STEM-MB-3596-LGZ)
Analysis of INPP5E Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1992-LGZ)
Analysis of Mycobacterium Paratuberculosis by Real-Time PCR Method (CAT#: STEM-MB-2946-LGZ)