Analysis of European Brown Hare Syndrome Virus (EBHSV) by Real-Time PCR Method (CAT#: STEM-MB-3865-LGZ)

Introduction

European Brown Hare Syndrome (EBHS) is a viral disease caused by a non-enveloped positive-strand RNA virus with a diameter of about 32–35 nm, belonging to the genus lagovirus of the Caliciviridae family. EBHS virus (EBHSV) is antigenically and genetically related to Rabbit Hemorrhagic Disease virus (RHDV).of rabbits (Oryctolagus cuniculus).<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.