Analysis of Eurytrema Coelomaticum by Real-Time PCR Method (CAT#: STEM-MB-3642-LGZ)

Introduction

The digenetic trematode Eurytrema coelomaticum is found parasitizing the pancreas and bile ducts of cattle herds and is occasionally found in humans. The parasite causes serious damage to the infected organ and causes significant economic losses.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.