Analysis of EXO1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0948-LGZ)

Introduction

Official Full Name: exonuclease 1
Also known as: HEX1; hExoI
This gene encodes a protein with 5' to 3' exonuclease activity and RNase H activity. It is similar to the S. cerevisiae protein Exo1 that interacts with Msh2 and is involved in mismatch repair and recombination. Alternative splicing of this gene produces three transcript variants encoding two different isoforms.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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