Analysis of Extracellular hemoglobin by UV-Vis Spectroscopy (CAT#: STEM-MB-0883-WXH)

Introduction

Extracellular hemoglobin, resulting from hemolysis or exogenous infusion, exerts toxic effects that are major components in the pathogenesis of many diseases and iatrogenic situations, including hemolytic anemias and transfusion-induced intravascular hemolysis, preeclampsia, intraventricular hemorrhage, sickle cell disease and chronic inflammatory leg ulcers, and infusion of recombinant hemoglobin. The pathogenesis involves one-electron reactions between oxy-Hb, its downstream metabolites heme, iron, ROS and free radicals (NO, superoxide etc.) and exposed tissue components. Besides, release of extracellular hemoglobin can result in the removal of beneficial free radical species (e.g. nitric oxide) and/or the production of reactive free radicals on the globin protein itself.