Analysis of F11R Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0946-LGZ)

Introduction

Official Full Name: F11 receptor<br />Also known as: JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1<br />Tight junctions are a mode of intercellular adhesion of epithelial or endothelial cell sheets that form a continuous seal around the cell and serve as a physical barrier preventing the free passage of solutes and water through the paracellular space. This immunoglobulin superfamily gene member encodes a protein that is an important regulator of tight junction assembly in epithelial cells. In addition, the encoded protein may serve as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1 involved in leukocyte trafficking, and (3) a platelet receptor. Multiple 5' alternatively spliced variants encoding the same protein have been identified, but their biological validity has not been determined.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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