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Analysis of FAP-α/SEPR (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0166-LGZ)

Introduction

Fibroblast activation protein, alpha (FAP-alpha) is a serine proteolytic enzyme with post-prolyl dipeptidyl peptidase activity and endopeptidase activity, and is activated by cleavage at the amino-terminal Xaa-Pro sequence. Involved in extracellular matrix degradation and involved in a variety of cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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