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Analysis of FasL (Human) by ELISA (CAT#: STEM-MB-0806-LGZ)

Introduction

FasL is a cytokine that can bind to the death receptor TNFRSF6/FAS, and mediates its apoptosis due to cytotoxicity during T-cell development.
Fas and its ligand FasL are the membrane surface molecules related to cell apoptosis that have been studied most deeply in recent years. The elucidation of their mechanism of action in apoptosis has had a profound impact on the in-depth understanding of the mechanism of cell apoptosis.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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