Analysis of FASLG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0965-LGZ)

Introduction

Official Full Name: Fas ligand<br />Also known as: APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A<br />This gene is a member of the tumor necrosis factor superfamily. The main function of the encoded transmembrane protein is to induce apoptosis by binding to FAS. The FAS/FASLG signaling pathway is critical for immune system regulation, including T cell activation-induced cell death (AICD) and cytotoxic T lymphocyte-induced cell death. It has also been linked to the development of several types of cancer. A defect in this gene may be associated with some cases of systemic lupus erythematosus (SLE). Alternative spliced transcript variants have been described.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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