Analysis of FBLIM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1310-LGZ)

Introduction

Official Full Name: filamin binding LIM protein 1
Also known as: CAL; FBLP1; FBLP-1
This gene encodes a protein with an N-terminal silk protein-binding domain, a proline-rich central domain, and multiple C-terminal LIM domains. This protein localizes at cell junctions, possibly linking cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin filaments and may play a role in regulating cell adhesion, cell morphology and cell motility. This protein is also localized in the nucleus and may affect cardiomyocyte differentiation after binding to the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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