Analysis of FBXO2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1309-LGZ)

Introduction

Official Full Name: F-box protein 2
Also known as: FBG1; FBX2; Fbs1; OCP1; NFB42
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins constitute one of the four subunits of the ubiquitin-protein ligase complex SCFs (SKP1-cullin-F-box) and function in phosphorylation-dependent ubiquitination. F-box proteins were classified into 3 classes: Fbws containing the WD-40 domain, Fbws containing leucine-rich repeats, and Fbxs containing different protein interaction modules or without recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to rat NFB42 (neural F Box 42 kDa) protein, which is enriched in the nervous system and may play a role in maintaining the post-mitotic state of neurons.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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