Analysis of FCRL5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0980-LGZ)

Introduction

Official Full Name: Fc receptor like 5<br />Also known as: CD307; FCRH5; IRTA2; BXMAS1; CD307e; PRO820<br />This gene encodes a member of the immunoglobulin receptor superfamily and fc receptor-like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-generation type I membrane protein containing eight immunoglobulin-like C2-type domains. This gene is related to B cell development and lymphomagenesis. Alternative splicing transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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