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Analysis of FGF acidic / FGF1 / FGFA by ELISA (CAT#: STEM-MB-1553-LGZ)

Introduction

The acid fibroblast growth factor (FGF acidic), also known as FGF-1, ECGF and HBGF-1, is a secreted type of fibroblast, which stimulates the proliferation of all cells and cells of the mesodermal source and the cells of the external embryo layer, the external embryo layer and the endodermal layer. It works in development, regeneration and angiogenesis. The internated FGF box can be transferred from the hsp90 to the body and then migrated to the nucleus by its two nuclear identification signals. The FGF, phosphorylated by pkcglyphosphorylation, can be triggered to output to the cytoplasm, where phosphorylation and degradation are applied here. In cells, FGF is the function of survival factors by inhibiting p53 activity and the transmission of apoptotic signals.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

FGF acidic / FGF1 / FGFA works in development, regeneration and angiogenesis.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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