Analysis of FGF (acidic) (Human) by ELISA (CAT#: STEM-MB-0809-LGZ)

Introduction

Acidic fibroblast growth factor, also known as FGF-1, ECGF, and HBGF-1, is a secretory mitogen that can stimulate the proliferation of all cells of mesodermal origin and cells of neuroectodermal, ectodermal, and endodermal origin. It plays a role in development, regeneration and angiogenesis. Internalized FGF acidic can be transferred to the cytoplasm with the help of Hsp90 and then migrated to the nucleus via its two nuclear localization signals. FGF acidic after PKCδ phosphorylation triggers active output into the cytoplasm, where it is dephosphorylated and degraded. In the cell, FGF acidic acts as a survival factor by inhibiting p53 activity and promoting apoptotic signaling.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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