Analysis of FGFR2 by ELISA (CAT#: STEM-MB-1518-LGZ)

Introduction

Fibroblast growth factor receptor 2 (FGFR2) is a tyrosine kinase receptor. FGFR1, FGFR2, FGFR3, and FGFR4 are transmembrane proteins, including extracellular, transmembrane, intracellular near-membrane and intracellular structural regions (tyrosine kinase regions), which provide the molecular basis of the receptor for specific binding of ligands. The Fibroblast growth factor receptor (FGFR) family promotes cell growth, enhances cell invasiveness and viability, and promotes angiogenesis. Among them, abnormal expression of human fibroblast growth factor 2 (FGFR2) and gene polymorphism are associated with a variety of tumors, such as breast cancer, bile duct cancer, lung cancer, and tumor cell proliferation and invasion

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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