Analysis of FMOD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0997-LGZ)

Introduction

Official Full Name: fibromodulin
Also known as: FM; SLRR2E
Fibromodulin belongs to the family of small interstitial proteoglycans. The central region of the encoded protein contains leucine-rich repeats with four keratin sulfate chains flanked by terminal domains containing disulfide bonds. The encoded protein may play a role in the assembly of the extracellular matrix due to interactions with type I and type II collagen fibrils and inhibition of fibrogenesis in vitro. It may also regulate TGF-β activity by sequestering TGF-β into the extracellular matrix. The sequence variation of this gene may be related to the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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