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Analysis of FN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1511-LGZ)

Introduction

Official Full Name: fibronectin 1
Also known as: FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
This gene encodes fibronectin, a glycoprotein that exists as a soluble dimer in plasma and as a dimer or multimer on the cell surface and in the extracellular matrix. The encoded preproprotein undergoes proteolytic processing to generate the mature protein. Fibronectin is involved in cellular adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense and metastasis. The gene has three alternatively spliced regions with the potential to generate 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Glomerulopathy with fibronectin deposits 2

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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