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Analysis of Folate R alpha (Human) by ELISA (CAT#: STEM-MB-0826-LGZ)

Introduction

Folate is an essential component of cellular metabolism and DNA synthesis and repair. It participates in the necessary one-carbon transfer reaction and is a vitamin required by both normal and tumor cells. Folate enters cells through two distinct systems: the reduced folate carrier and the folate receptor system utilizing a bidirectional anion exchange mechanism. Folate Receptor alpha (Folate R alpha) is a membrane-bound member of the folate receptor family that facilitates folate transport through a mechanism called phagocytosis, in which the receptor is internalized and then recycled back to the cell membrane.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: serum, plasma, tissue homogenates
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