Analysis of Follistatin (Human) by ELISA (CAT#: STEM-MB-0827-LGZ)

Introduction

Follistatin, also known as FSH inhibitory protein, is a single-chain glycoprotein. Follistatin, as the binding protein of Activin (ACT), regulates the reproductive activity of animals through the Activin/Follistatin system. Follistatin combines with many members of the transforming growth factor-13 (TGF-β) superfamily in a paracrine or autocrine manner, such as BMP, bone morphognic protein, myostatin, etc., and plays an important role in organs and tissues other than the reproductive system. Moreover, research results have found that it has an important regulatory effect on animal muscle growth. Follistatin can bind to the C-terminus of myostatin, thereby inhibiting the binding of myostatin to receptors and promoting skeletal muscle development.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Muscle Injury, Immunology/Inflammation, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

Other recommended products

Analysis of Human VDR(Vitamin D Receptor) by ELISA (CAT#: STEM-MB-1840-LGZ)
Analysis of Plant Gibberellic Acid 3 (GA3) by ELISA (CAT#: STEM-MB-2263-LGZ)
Analysis of Rat COMP(Cartilage Oligomeric Matrix Protein) by ELISA (CAT#: STEM-MB-2016-LGZ)
Analysis of Mouse SLC(Secondary Lymphoid Tissue Chemokine) by ELISA (CAT#: STEM-MB-1920-LGZ)
Analysis of Calprotectin (Human) by ELISA (CAT#: STEM-MB-0712-LGZ)
Analysis of Human GR(Glutathione Reductase) by ELISA (CAT#: STEM-MB-1775-LGZ)
Analysis of TSLP (Human) by ELISA (CAT#: STEM-MB-1249-LGZ)
Analysis of IL-12p70 (Human) by ELISA (CAT#: STEM-MB-0935-LGZ)