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Analysis of FRS2 (Human, Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0193-LGZ)

Introduction

Fibroblast growth factor receptor substrate 2 (FRS2) enables fibroblast growth factor receptor binding activity and neurotrophic factor TrkA receptor binding activity. Can participate in the negative regulation of cardiomyocyte differentiation. And can act on the upstream or internal of the fibroblast growth factor receptor signaling pathway. It is a biomarker for renal cell carcinoma.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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