Analysis of Fusarium Poae by Real-Time PCR Method (CAT#: STEM-MB-3435-LGZ)

Introduction

Fusarium poae is a necrotrophic pathogen able to produce several mycotoxins not only type A such as HT-2, T-2 and diacetoxyscirpenol (DAS) and B trichothecenes such as nivalenol (NIV) with harmful effects on human and animal health but also other minor mycotoxins such as enniatins, beauvericin, neosolaniol with minor effects on consumers but equally important.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.