Analysis of β - galactosidase (β –GAL) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1389-LGZ)

Introduction

Sensitivity: 16.19 U/L
Detection range: 16.19-1200.0 U/L
Precision: inter-lot difference of 6%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 390-410 nm

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

β-galactosidase can catalyse substrate PNPG (p-nitrophenyl-β-D-galactosidine) to p-nitrophenol. P-nitrophenol has a maximum absorption peak at 400 nm. The enzyme activity of β-GAL can be calculated by measuring the change of absorbance value in unit time.

Applications

It is suitable for the detection of β-galactosidase activity in serum (plasma), animal and plant tissues and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, animal and plant tissues and cell samples

Other recommended products

Analysis of Peroxidase (POD) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1434-LGZ)
Analysis of Glucose-6-phosphate (G6P) by Enzyme-labeled Instrument (CAT#: STEM-MB-1432-LGZ)
Analysis of Total Oxidant Status (TOS) by Enzyme-labeled Instrument (CAT#: STEM-MB-1449-LGZ)
Analysis of Total Antioxidant Capacity (T-AOC) by Enzyme-labeled Instrument (CAT#: STEM-MB-1390-LGZ)
Analysis of Total Bilirubin (TBIL) by Enzyme-labeled Instrument (CAT#: STEM-MB-1439-LGZ)
Analysis of Glutamine (Gln) by Enzyme-labeled Instrument (CAT#: STEM-MB-1470-LGZ)
Analysis of Glucose Uptake by Fluorescence Microplate Reader (CAT#: STEM-MB-1510-LGZ)
Analysis of CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1392-LGZ)