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Analysis of GCPII/PSMA (Human) by ELISA (CAT#: STEM-MB-0837-LGZ)

Introduction

Prostate-specific membrane antigen (PSMA) is a nonsecretory membrane enzyme with carboxypeptidase and folate hydrolase activities and a large extracellular domain. PSMA receptors have an oncogenic signaling role in prostate cancer cells, acting on glutamate receptors and activating Pi3K and Akt growth pathways. It is overexpressed in 90% of metastatic prostate cancers, with low physiological expression in normal tissues (prostate, small intestine, salivary and lacrimal glands, and kidney).




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Prostate Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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