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Analysis of GEN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1126-LGZ)

Introduction

Official Full Name: GEN1 Holliday junction 5' flap endonuclease
Also known as: Gen
This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in the breakdown of Holliday junctions, intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. This protein breaks down holiday junctions by forming a double nick at the junction, resulting in a gapped duplex product that can be ligated. Furthermore, this protein has been found to localize to the centrosome, where it is involved in the regulation of centrosome integrity. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary cancer-predisposing syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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