Analysis of Gene Expression by RT-qPCR (CAT#: STEM-MB-0229-WXH)

Introduction

When studying gene expression with real-time polymerase chain reaction (PCR), scientists usually investigate changes – increases or decreases – in the expression of a particular gene or set of genes by measuring the abundance of the gene-specific transcript.
Reverse transcription quantitative PCR (RT-qPCR) is considered today as the gold standard for accurate, sensitive and fast measurement of gene expression. It is a fast and convenient PCR method that combines traditional RT-PCR with the phenomenon of fl uorescence resonance energy transfer (FRET) using fluorogenic primers. The detection of changes in fluorescence intensity during the reaction enables the user to follow the PCR reaction in real time.