Analysis of GFI1B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1969-LGZ)

Introduction

Official Full Name: growth factor independent 1B transcriptional repressor
Also known as: BDPLT17; ZNF163B
This gene encodes a zinc finger-containing transcriptional regulator that is predominantly expressed in cells of the hematopoietic lineage. The encoded protein binds to many other transcriptional regulators, including GATA-1, dwarf-associated transcription factor 1, and histone deacetylases, and controls the expression of genes involved in erythroid and megakaryocyte development and maturation. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet hemorrhagic disorder-17. Alternate splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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