Analysis of GGPS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0989-LGZ)

Introduction

Official Full Name: geranylgeranyl diphosphate synthase 1<br />Also known as: GGPPS; MDHLO; GGPPS1; MUDHLOV<br />This gene is a member of the pentenyltransferase family and encodes a protein with geranyl diphosphate synthase activity. This enzyme catalyzes the synthesis of GGPP from farnesyl diphosphate and isopentenyl diphosphate. GGPP is an important molecule responsible for the C20-enoylation of proteins and the regulation of nuclear hormone receptors. Protein-coding and non-protein-coding transcriptional splice variants have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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