Analysis of GIGYF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1539-LGZ)

Introduction

Official Full Name: GRB10 interacting GYF protein 2
Also known as: GYF2; PERQ2; PERQ3; PARK11; TNRC15
The gene contains CAG trinucleotide repeats and encodes a protein containing stretches of polyglutamine residues. The encoded protein may be involved in the regulation of tyrosine kinase receptor signaling. The gene is located in the region of the chromosome associated with the inheritance of Parkinson's disease type 11, and mutations in this gene are thought to cause the disease. However, recent studies in different populations have been unable to replicate this association. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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