Analysis of GJA5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0988-LGZ)

Introduction

Official Full Name: gap junction protein alpha 5
Also known as: CX40; ATFB11
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of BPNT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0796-LGZ)
Analysis of Trypanosoma Cruzi by Real-Time PCR Method (CAT#: STEM-MB-3568-LGZ)
Analysis of Human Herpesvirus-6A (HHV-6A) by Real-Time PCR Method (CAT#: STEM-MB-3326-LGZ)
Analysis of SHISA4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0659-LGZ)
Analysis of Histoplasma Capsulatum Var. Capsulatum by Real-Time PCR Method (CAT#: STEM-MB-3818-LGZ)
Analysis of B3GALT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0172-LGZ)
Analysis of Human Immunodeficiency Virus 1 (HIV-1M-K) by Real-Time PCR Method (CAT#: STEM-MB-3222-LGZ)
Analysis of Schistosoma Incognitum by Real-Time PCR Method (CAT#: STEM-MB-3361-LGZ)