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Analysis of Glycerol by Enzyme-labeled Instrument (CAT#: STEM-MB-1472-LGZ)

Introduction

Sensitivity: 0.01mmol/L
Detection range: 0.01-1.00 mmol/L
Precision: inter-lot difference of 8%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 500-520 nm




Principle

Glycerol is converted by enzyme to produce hydrogen peroxide. In the presence of 4-amino-antipyrine and phenol, hydrogen peroxide is catalyzed by oxidase to produce red quinone compounds, the color of which is proportional to the glycerol content.

Applications

It is used to detect the glycerol content in serum, plasma, animal and plant tissues and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, animal and plant tissues and cell samples
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