Analysis of GOLPH3L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1012-LGZ)

Introduction

Official Full Name: golgi phosphoprotein 3 like
Also known as: GPP34R
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is localized at the Golgi stack and may have a regulatory role in Golgi trafficking.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Porcine Rotavirus by Real-Time PCR Method (CAT#: STEM-MB-3328-LGZ)
Analysis of PRAMEF11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1385-LGZ)
Analysis of EFNA3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0932-LGZ)
Analysis of Candida Tropicalis by Real-Time PCR Method (CAT#: STEM-MB-2701-LGZ)
Analysis of ASH2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1895-LGZ)
Analysis of CALM2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1724-LGZ)
Analysis of Trichuris Suis by Real-Time PCR Method (CAT#: STEM-MB-3765-LGZ)
Analysis of DUSP23 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0908-LGZ)