Analysis of gp130 (soluble) (Human) by ELISA (CAT#: STEM-MB-0866-LGZ)

Introduction

gp130 is a 130 kD glycoprotein, which is the signal transduction chain of interleukin-6 (IL-6). The binding of IL-6 to IL-6 receptor (IL-6R) does not cause signal transmembrane transmission, but must bind to two gp130 molecules for homodimerization, which leads to the activation of Jak kinase in cytoplasm and causes signal transmission. The gp130 molecule is expressed in membrane form outside the cell membrane and is also found to exist in soluble form (sgp130) in plasma.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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