Analysis of GPA33 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1008-LGZ)

Introduction

Official Full Name: glycoprotein A33<br />Also known as: A33<br />The glycoprotein encoded by this gene is a cell surface antigen expressed in more than 95% of human colon cancers. The open reading frame encodes a 319 amino acid polypeptide with a putative secretion signal sequence and three potential glycosylation sites. The predicted mature protein has a 213 amino acid extracellular domain, a transmembrane domain and a 62 amino acid intracellular tail. The extracellular domain sequence contains two domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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