Analysis of GPR137B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1005-LGZ)

Introduction

Official Full Name: G protein-coupled receptor 137B<br />Also known as: TM7SF1<br />Involved in several processes, including positive regulation of TORC1 signaling; positive regulation of protein localization to lysosome; and regulation of GTPase activity. Located in lysosomal membrane.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Leishmania Infantum by Real-Time PCR Method (CAT#: STEM-MB-3122-LGZ)
Analysis of DISP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0918-LGZ)
Analysis of C1orf116 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0240-LGZ)
Analysis of Simian T Lymphotropic Virus type 1 (STL V-1) by Real-Time PCR Method (CAT#: STEM-MB-3856-LGZ)
Analysis of SPMIP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0242-LGZ)
Analysis of NLRP6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2326-LGZ)
Analysis of KRTAP5-3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2281-LGZ)
Analysis of CNGA3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1446-LGZ)