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Analysis of Green synthesis of silver nanoparticles using Capsicum annuum L. extract by UV-Vis Spectroscopy (CAT#: STEM-MB-0996-WXH)

Introduction

A number of approaches are available for the synthesis of silver NPs. For example, silver ions are reduced by chemical, electrochemical, radiation, and photochemical methods, and Langmuir–Blodgett, and biological techniques. Among those methods, biological syntheses are not only a good way to fabricate benign nanostructure materials, but also to reduce the use or generation of hazardous substances to human health and the environment.
Capsicum annuum L. is a traditional vegetable in China and northern America, and is largely cultivated in many countries. It is available in supermarkets and is inexpensive. In addition, no toxicity, compared to other reducing agents, makes it an ideal multifunctional agent for the production of nanomaterials. The Capsicum annuum L. extract contains a lot of biomolecules, such as proteins/ enzymes, polysaccharides, amino acids and vitamins, et al.,which could be used as reductants to react with silver ions and as scaffolds to direct the formation of silver NPs in solution.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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