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Analysis of GREM2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1029-LGZ)

Introduction

Official Full Name: gremlin 2, DAN family BMP antagonist
Also known as: PRDC; DAND3; STHAG9; CKTSF1B2
This gene encodes a member of the BMP (bone morphogenetic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and often form homo- and heterodimers. The gene belongs to the CAN (cerberus and dan) subfamily of BMP antagonists and is characterized by a C-terminal cystine knot and an eight-membered loop. Antagonism of the secreted glycosylated protein encoded by this gene may be due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning and tissue differentiation.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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