Analysis of GRN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2481-LGZ)

Introduction

Official Full Name: granulin precursor
Also known as: GEP; GP88; PEPI; PGRN; CLN11; PCDGF
Granulins are a family of secreted glycosylated peptides that are cleaved from a single precursor protein of 7.5 repeats of a highly conserved 12-cysteine granulin/epithelial motif. 88 kDa precursor protein, progranulin, also known as proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide yields mature granulin, which can be further cleaved into various active 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelium 1 and Epithelium 2 are synonymous with granulin A and B, respectively. Both polypeptides and intact granules regulate cell growth. However, different members of the granulin family may play inhibitory, stimulatory or dual roles on cell growth. Members of the granulin family play important roles in normal development, wound healing, and tumorigenesis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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