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Analysis of GRO-α (Non-human primate) by ELISA (CAT#: STEM-MB-0873-LGZ)

Introduction

CXCL1 (chemokine (C-X-C motif) ligand 1), also known as GRO-α, NAP-3, or MGSA, belongs to the CXC chemokine subfamily and is a polypeptide originally isolated from human melanoma cells. CXCL1 is involved in the development of many inflammatory diseases, including the induction of angiogenesis and the recruitment of neutrophils. CXCL1 is produced by several cell types and activates CXCR2 as well as high levels of CXCR1. CXCL1 also regulates angiogenesis, tumorigenesis and wound healing.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma, Serum
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