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Analysis of GSTM3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1195-LGZ)

Introduction

Official Full Name: glutathione S-transferase mu 3<br />Also known as: GST5; GSTB; GTM3; GSTM3-3; GSTM3TV2; hGSTM3-3<br />The cytoplasmic and membrane-bound forms of glutathione S-transferase are encoded by two distinct epigene families. Currently, eight different classes of soluble mammalian cytoplasmic glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta, and zeta. This gene encodes glutathione s-transferase and belongs to the mu class. This class of enzymes detoxifies electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress, by conjugating glutathione. Genes encoding mu-like enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can alter an individual's susceptibility to carcinogens and toxins, and can also affect the toxicity and efficacy of certain drugs. Mutations in this class of mu genes are associated with a slight increase in some cancers, possibly due to exposure to environmental toxins. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements