Analysis of H+K+-ATPase by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1353-LGZ)

Atpase can decompose ATP to produce ADP and inorganic phosphorus. The activity of the enzyme can be expressed by measuring the production amount of inorganic phosphorus in unit time. Inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium phosphomolybdate complex, which is reduced by reducing agent to form molybdenum blue, and has an absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

It is used to detect the activity of hydropotassium ATPase in animal tissues and cultured cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissues and cultured cells