Analysis of H2AC21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1024-LGZ)

Introduction

Official Full Name: H2A clustered histone 21
Also known as: H2AB; HIST2H2AB
Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). By linking DNA interactions between histone H1 and nucleosomes, chromatin fibers are further compacted to form higher-order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the H2A histone family. The transcript of this gene contains a palindromic termination element.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ATP1A4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0780-LGZ)
Analysis of Turkey Astrovirus (TAstV) by Real-Time PCR Method (CAT#: STEM-MB-3665-LGZ)
Analysis of Rotavirus A by Real-Time PCR Method (CAT#: STEM-MB-3157-LGZ)
Analysis of Neisseria Spp. by Real-Time PCR Method (CAT#: STEM-MB-3082-LGZ)
Analysis of ALX3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1177-LGZ)
Analysis of Avian Infectious Bronchitis Virus 793B Type (AIBV-793B) by Real-Time PCR Method (CAT#: STEM-MB-2863-LGZ)
Analysis of Leishmania Tropica by Real-Time PCR Method (CAT#: STEM-MB-3124-LGZ)
Analysis of MTUS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1912-LGZ)