Analysis of H2BC18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1022-LGZ)

Introduction

Official Full Name: H2B clustered histone 18
Also known as: HIST2H2BF
Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. The structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of each pair of four core histones (H2A, H2B, H3, and H4). By linking DNA interactions between histone H1 and nucleosomes, chromatin fibers are further compacted to form higher-order chromatin structures. This gene encodes a replication-dependent histone, a member of the histone H2B family, present in a histone cluster on chromosome 1.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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