Analysis of H3C13 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1017-LGZ)

Introduction

Official Full Name: H3 clustered histone 13
Also known as: H3C14; H3C15; HIST2H3D
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Aeromonas Punctata by Real-Time PCR Method (CAT#: STEM-MB-3292-LGZ)
Analysis of W-CSFV Ag by Real-Time PCR Method (CAT#: STEM-MB-2927-LGZ)
Analysis of SDPR Expression by RT-qPCR (CAT#: STEM-MT-0064-LGZ)
Analysis of IRF6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1057-LGZ)
Analysis of FCGR1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0960-LGZ)
Analysis of ERLIN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2100-LGZ)
Analysis of SNED1-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1668-LGZ)
Analysis of KIT and PDGFRA Somatic Mutations in all GIST samples by RT-qPCR (CAT#: STEM-MT-0002-LGZ)